Signalling

Part:BBa_J46000:Design

Designed by: Graham Wiley   Group: iGEM06_Oklahoma   (2006-08-11)


Arabinose Sensor linked to ECFP and HSL production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2623
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Arabinose was used as a trigger for this part since it is necessary to grow E.coli on a media without arabinose.


Source

iGEM 2006 parts

References